Three of the most common metrics in drug discover are Kd, IC50 and EC50. At first glance it can seem that they measure the same thing, but they don’t. Kd measures how tightly a molecule or compound binds to its target. IC50 measures inhibition of a function and conversely, EC50 measures activation or induction of a response. Confusing these values can lead to misinterpretation of assay results and costly rework. Let’s take a closer look at each one.
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