Rapid Test to Detect SARS-CoV-2 Developed in Brazil

Posted on by Michele Arduengo

The Virology lab at the Universidade Federal da Bahia (UFBA), led by Dr. Gúbio Soares, has developed a fast and specific real-time PCR assay using GoTaq 1-Step RT-qPCR for detection of SARS-2-CoV (the coronavirus previously named 2019-nCoV), which causes the respiratory disease COVID-19. The Maxwell RSC instrument is used for automated extraction of RNA from oral-pharyngeal secretion collected by swab or bronchial wash prior to the assay. This coronavirus-specific assay can shorten the time to identify SARS-2-CoV from 48 hours to 3 hours (1), providing critical information to public health officials in a timely fashion.

“Promega has been providing all our reagents for standard and real-time PCR and also for nucleic acid extraction. It’s a company I can rely on the relationship; they are our partners and have provided excellent support both technically and financially. Promega is the base of all our assays.” Dr. Gúbio Soares.  

Dr. Soares’ laboratory has experience developing assays to identify and detect emerging viral pathogens. Their laboratory first identified the Zika virus as the etiologic agent in the large outbreaks of acute exanthematous illness (AEI) in northeast Brazil in April 2015 (2). Zika was eventually declared a public health emergency of international importance by the World Health Organization in February 2016, after increased incidence of microcephaly was detected in the infants of women infected during pregnancy. Many of the lessons learned in the management of the Zika crisis are informing how scientists are addressing SARS-2-CoV. The Zika response was characterized by a collaborative spirit to share data, samples and resources among scientific labs across the globe.

Below is a video link from Brazil (audio in Portuguese) describing Dr. Soares’ group work on SARS-2-CoV. https://globoplay.globo.com/v/8302334/

Sources Cited

  1. TV Bahia (2020) Test developed at UFBA can identify coronavirus in 3 hours, says researcher. [Internet: https://g1.globo.com/ba/bahia/noticia/2020/02/07/teste-desenvolvido-na-ufba-pode-identificar-coronavirus-em-3h-diz-pesquisador.ghtml Accessed: February 19, 2020]
  2. Sandler, N. (2016) Zika: Personal Perspectives, Global Responses Promega Corporation. [Internet: Accessed : February 19, 2020]

Related Posts

From Forensic Analysis to Taco Thursday: My Experience as a Promega Intern

Posted on by Promega

Today’s blog is written by guest blogger, Kali Denis, an intern in our scientific applications group. You’ll find her bio at the end of the article.

A few months ago, I stood in front of my freezer at home, holding a bag with a tube full of gum that I chewed. The freezer was overflowing, as we had just done our weekly grocery shopping, so I ended up stuffing the bag next to some frozen fish sticks. I wondered how long it would take for one of my roommates to question just exactly what this gross-looking bag was doing in our freezer. I doubt they would have ever guessed that it was for a project at my internship!

Continue reading “From Forensic Analysis to Taco Thursday: My Experience as a Promega Intern”

Researching the Researcher: Abbeah Navasca, 2019 Real-Time PCR Grant Winner

Posted on by Promega

The three winners of the 2019 Real-Time PCR Grants have been hard at work in the six months since receiving their grants. Each winner was eligible to receive up to $10,000 in free PCR reagents as well as the opportunity to collaborate with our knowledgeable technical service and training teams.

Abbeah Navasca is a plant pathology researcher with the Tagum Agricultural Development Company, Inc. (TADECO*, Philippines). She is developing treatments for viral infections that affect one of Philippines’ largest and most valuable agricultural exports: bananas. As a result of the qPCR grant, she and two of her colleagues were able to participate in sample preparation and analysis workshops with Promega Technical Services experts in Singapore. During her visit, the team worked through strategies for plant sample preparation and amplified those samples with the GoTaq® 1-Step RT-qPCR System. We had a chance to ask her more before she headed back to her lab.

Continue reading “Researching the Researcher: Abbeah Navasca, 2019 Real-Time PCR Grant Winner”

Tidying Up With Promega R&D: Spectrometer From Space?

Posted on by Jordan Villanueva

Later this year, Promega will open a new R&D building with more than twice the current amount of lab space available on the Madison campus. While preparing to move to the new building, R&D scientists are cleaning out decades of scientific history housed in some of the older labs. Meagan Eggers, Promega Strategic Information Partner, is collaborating with the research groups to document and preserve noteworthy artifacts unearthed in the Research & Development Center. Over the next few months, we’ll showcase some of the most interesting things we find.

Spectrometer – 1960s-2000

Promega research scientists began investigating bioluminescent proteins in the early 1990s. One of the most important tools in this research was the spectrometer pictured above, which was used to measure the emission spectra of many different organisms. Before it arrived at Promega, however, this spectrometer began in the space program.

Continue reading “Tidying Up With Promega R&D: Spectrometer From Space?”

Investigation of Remdesivir as a Possible Treatment for SARS-CoV-2 (2019-nCoV)

Posted on by Kyle Hooper

Remdesivir (RDV or GS-5734) was used in the treatment of the first case of the SARS-CoV-2 (formerly 2019-nCoV ) in the United States (1). RDV is not an approved drug in any country but has been requested by a number of agencies worldwide to help combat the SARS-CoV-2 virus (2). RDV is an adenine nucleotide monophosphate analog demonstrated to inhibit Ebola virus replication (3). RDV is bioactivated to the triphosphate form within cells and acts as an alternative substrate for the replication-necessary RNA dependent RNA polymerase (RdRp). Incorporation of the analog results in early termination of the primer extension product resulting in the inhibition.

Note the spikes that adorn the outer surface of the virus, which impart the look of a corona surrounding the virion, when viewed electron microscopically. In this view, the protein particles E, S, M, and HE, also located on the outer surface of the particle, have all been labeled as well. A novel coronavirus virus was identified as the cause of an outbreak of respiratory illness first detected in Wuhan, China in 2019.
This illustration, created at the Centers for Disease Control and Prevention (CDC), reveals ultrastructural morphology exhibited by coronaviruses. Photo Credit: Alissa Eckert, MS; Dan Higgins, MAM CDC

Why all the interest in RDV as a treatment for SARS-CoV-2 ? Much of the interest in RDV is due to a series of studies performed by collaborating groups at the University of North Carolina Chapel Hill (Ralph S. Baric’s lab) and Vanderbilit University Medical Center (Mark R. Denison’s lab) in collaboration with Gilead Sciences. 

Continue reading “Investigation of Remdesivir as a Possible Treatment for SARS-CoV-2 (2019-nCoV)”

Producing Snake Venom— in the Lab

Posted on by Johanna Lee

Snakebite is a serious public health issue in many tropical countries. Every year, roughly 2 million cases of poisoning from snakebites occur, and more than 100,000 people die. Snake venom is extremely complex, containing a cocktail of chemicals, many of which are undefined. This complicates the development of new therapeutics for treating snakebite.

Antivenom is the most effective treatment for snakebites, but its production is complex and dangerous. It involves manually milking the venom from different species of live snakes, then injecting small doses of the venom into animals (mostly horses) to stimulate an immune response. After a period of time, antibodies form in the animal’s blood, which is purified for use as antivenom.

But what if we could produce snake venom in the lab, instead of using live snakes? Recently, a group from the Netherlands did just that by growing organoids derived from snake venom glands.

Continue reading “Producing Snake Venom— in the Lab”

The Race to Develop New Therapeutics Against Coronaviruses

Posted on by Michele Arduengo

Once the purview of virology researchers, the word “coronavirus” is now part of the vernacular in the mainstream media as reports of quarantined cruise ships (1) and makeshift hospitals (2) fill our online news feeds. While there is currently no approved anti-viral treatment for coronavirus infection (3), a team led by researchers from Vanderbilt University recently published work characterizing the anti-CoV activity of a compound, which they now plan to test against 2019-nCoV (4).

Developing New Therapeutics Against Coronaviruses

Coronaviruses (CoVs) are enveloped, single-stranded RNA viruses that exhibit cross-species transmission—the ability to spread quickly from one host (e.g., civet) to another (e.g., human). Scientists classify CoVs into four groups based on the nature of the spikes on their surface: alpha (α), beta (ß), gamma (γ) and delta (δ, 1). Only the alpha- and beta-CoVs can infect humans. Four coronaviruses commonly circulate within human populations: Human CoV 229E (HCoV229E), HCoVNL63, HCoVOC43, and HCoVHKU1. Three other CoVs have emerged as infectious agents, jumping from their normal animal host species to humans: SARS-CoV, MERS-CoV and most recently, 2019-nCoV (5).

TE micrograph of a single MERS-CoV
Digitally colorized transmission electron micrograph reveals ultrastructural details of a single Middle East respiratory syndrome coronavirus (MERS-CoV) virion. Image credit: National Institute of Allergy and Infectious Diseases

The need for an effective, broad spectrum treatment against HCoVs, has been brought into sharp focus by the recent outbreak of the 2019 Novel Coronavirus (2019-nCoV; 6).

Continue reading “The Race to Develop New Therapeutics Against Coronaviruses”

How Does Our Garden Grow?

Posted on by Darcia Schweitzer

It’s the time of year in the northern US when you start to the miss green grass, ample daylight and warm breezes that are still months away. The promise of spring’s renewal and seedlings sprouting from the snow-covered ground seems too far out to even indulge in a daydream of better weather.

But then again, I’m not a farmer.                                                    

The Promega Culinary Garden at Bluebird Farms.

Now is the time of year when farmers are reflecting on last year’s harvest, making decisions about changes that need to be made and planning for the upcoming growing season. This work includes choosing what plants and varieties will be planted, estimating how many of each are needed and ordering the seeds. Crop rotation and cover crops are also part of the considerations.

If you’re a regular reader of this blog, you may know that Promega has a culinary garden that supplies some of the produce for our cafeterias on the Madison campus. During the growing season our Culinary Gardener, Logan Morrow, oversees the operations of Bluebird Farms with the help of his colleage Mike Daugherty.

Continue reading “How Does Our Garden Grow?”

Targeted Protein Degradation: A Bright Future for Drug Discovery

Posted on by Ken Doyle
targeted protein degradation and protacs

Our cells have evolved multiple mechanisms for “taking out the trash”—breaking down and disposing of cellular components that are defective, damaged or no longer required. Within a cell, these processes are balanced by the synthesis of new components, so that DNA, RNA and proteins are constantly undergoing turnover.

Proteins are degraded by two major components of the cellular machinery. The discovery of the lysosome in the mid-1950s provided considerable insight into the first of these degradation mechanisms for extracellular and cytosolic proteins. Over the next several decades, details of a second protein degradation mechanism emerged: the ubiquitin-proteasome system (UPS). Ubiquitin is a small, highly conserved polypeptide that is used to selectively tag proteins for degradation within the cell. Multiple ubiquitin tags are generally attached to a single targeted protein. This ill-fated, ubiquitinated protein is then recognized by the proteasome, a large protein complex with proteolytic activity. Ubiquitination is a multistep process, involving several specialized enzymes. The final step in the process is mediated by a family of ubiquitin ligases, known as E3.

Continue reading “Targeted Protein Degradation: A Bright Future for Drug Discovery”

New Year, New Buildings, Same Commitment to Sustainability

Posted on by Jordan Villanueva

In times of rapid growth, we look to the future with excitement while also assuring that our expansion is sustainable. The Promega Global Facilities Planning Team emphasizes environmental stewardship and long-term planning. Each building is designed to meet ambitious sustainability goals, and innovations incorporated into each project inform the next. In 2019, we finished construction on two new buildings in Europe and made progress on two important facilities at our headquarters in Wisconsin.

Continue reading “New Year, New Buildings, Same Commitment to Sustainability”