Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) by which antibodies target diseased cells for elimination. Traditional methods for measuring ADCC require primary donor peripheral blood mononuclear cells (PBMCs) or purified natural killer (NK) cells that express Fc receptors on the cell surface. Killing of target cells is an endpoint […]
Today’s blog is contributed by guest blogger Caitlin Cavanaugh, Client Support Consultant with Promega North America. Recently, I began a new role as a client support consultant at Promega. In this role, I’m responsible for all technical and sales support for the Promega portfolio in the New Jersey and Philidelphia area. Before coming to Promega, I worked in a lab at a start-up company […]
Kinase target engagement is a new way to study kinase inhibitors for target selectivity, potency and residency. The NanoBRET™ TE Intracellular Kinase Assays enable you to quantitate kinase-inhibitor binding in live cells, making these assays an exciting new tool for kinase drug discovery research. For today’s blog about NanoBRET™ TE Intracellular Kinase Assay, we feature spokesperson […]
“Today is certainly a great day for Promega R&D, but it is also a great day for science.” Gary Tarpley, director of Research and Development at Promega closed his remarks for the ground breaking of the new Promega Research and Development Center in Fitchburg, WI, with those words. With the ground breaking on this new […]
Late in 2017, a group here at Promega launched an exciting new assay, the NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay. It’s easy for me to call this assay exciting; I was an editor on the project team. But judging by the reviews on the SelectScience® web site, others are excited about NanoBRET™ Target Engagement […]
A recent paper in Journal of Medicinal Chemistry, “Discovery of GDC-0853: A Potent, Selective and Noncovalent Bruton’s Tyrosine Kinase Inhibitor in Early Clinical Development” (1) details some elegant work in chemical modification and extensive testing during exploration of inhibitors for BTK. As a warmup to the article, here is a brief BTK backstory. BTK (Bruton […]
This blog was originally published in April of 2018. This update includes the paper, “Quantitative, Wide-Spectrum Kinase Profiling in Live Cells for Assessing the Effect of Cellular ATP on Target Engagement” from Cell Chemical Biology, demonstrating the power of NanoBRET™ target engagement kinase assays in the study of kinase inhibitors. The review “Kinase Inhibitors: the […]
The Foundation for Food and Agriculture Research (FFAR) announced on November 30 that they are awarding $1M to a project based at the University of California, Davis, to study protein kinases of rice plants. The team is led by Dr. Pamela Ronald, a leading expert in plant genetics who has engineered disease- and flood-resistant rice. […]
When I was in grad school and pictured what a role in industry would look like, the first thing that came to my mind was a Research and Development (R&D) Scientist. My life as a grad student and as a postdoc revolved around benchwork, so that must be the case in industry too, right? It […]
Research surrounding drug discovery has historically been highly competitive and expensive. Unfortunately, many late-stage drug failures have occurred over recent years, often due to lack of efficacy. These failures have left the industry searching for new means by which to improve early drug discovery efforts aimed at understanding the drug target and its role in […]
