Finding a Cure for COVID-19: Spotlight on Virologist Dr. Colleen Jonsson

Photograph of Dr. Jonsson of UTSHC whose research includes finding small molecule antivirals for SARS-CoV-2
Dr. Colleen Jonsson, UTHSC

Since the COVID-19 pandemic swept the world in early 2020, many scientists in the viral research community have shifted their focus to study the SARS-CoV-2 coronavirus. Dr. Colleen Jonsson is one of them. She’s the Director of the Regional Biocontainment Laboratory, and Director of the Institute for the Study of Host-Pathogen Systems at the University of Tennessee Health Science Center (UTHSC) in Memphis.

Dr. Jonsson has been studying highly pathogenic human viruses for more than three decades. She has led several cross-institutional projects using high-throughput screens to discover small molecule antiviral compounds that could be used as therapeutics. And now, she’s using that experience to find an antiviral therapeutic against SARS-CoV-2.

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Reduce, Reuse, and Recycle During the Holidays

In the latter months of the year, the holiday season brings lots of gifts, packages, and deliveries. One of the best ways to spread holiday cheer and connect with one another is to reduce, reuse, and recycle packaging material. Doing so will benefit the gift that keeps on giving—our planet.

The question is, what’s the best way to reduce, reuse, and recycle the materials used for gifting? First, it’s important to know which gifting material is recyclable and which is not recyclable. 

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Biotechnology Teaching Online: A New Way to Look at Scientific Notebooks

This post is written by guest blogger, Peter Kritsch MS, Adjunct Instructor BTC Institute.

When I was in the middle of my junior year in high school, my family moved. We had lived in the first state for 12 years. I had gone to school there since kindergarten. Although it wasn’t a small district, I knew everybody and, for better or worse, everybody knew me. Often the first reaction I get when I tell people when we moved is that it must have been hard to move so close to graduation. The reality is . . . it really wasn’t. In fact, it was quite liberating. See, I didn’t have to live up to anybody else’s expectations of who I was based on some shared experience in 2nd grade. I had the opportunity to be who I wanted to be, to try new things without feeling like I couldn’t because that wasn’t who I was supposed to be. 

As long as I refrained from beginning too many sentences with “Well at my old school . . . “ people had to accept me for who I was in that moment, not for who they perceived me to be for the previous 12 years. Now, the new activities were not radically different. I still played baseball and still geeked out taking AP science classes, but I picked up new activities like golf, playing basketball with my friends, and even joined the yearbook. I know . . . “radically different.”  The point is that the new situation allowed me to try something new without worrying about what had always been. 

Peter teaches about biofuels in his virtual classroom.

The pandemic has forced a lot of us to move our classrooms online. In a short period of time, everything changed about how education was done. Our prior teaching experience, including the experience I had with doing blended learning (ooops . . . “back at my old school”), was helpful to a point.  But we quickly found out that being completely virtual was different. And as science teachers, how do you do more than just teach concepts when online? How do you help students to continue engaging in the crucial parts of science – observing, questioning, designing, analyzing, and communicating?

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Mass Spec for Glycosylation Analysis of SARS-CoV-2 Proteins Implicated in Host-Cell Entry

The spike protein of the SARS-CoV-2 virus is a very commonly researched target in COVID-19 vaccine and therapeutic studies because it is an integral part of host cell entry through interactions between the S1 subunit of the spike protein with the ACE2 protein on the target cell surface. Viral proteins important in host cell entry are typically highly glycosylated. Looking at the sequence of the SARS-CoV-2 virus, researchers predict that the spike protein is highly glycosylated. In a recent study, researchers conducted a glycosylation analysis of SARS-CoV-2 proteins using mass spec analysis to determine the N-glycosylation profile of the subunits that make up the spike protein.

3d model of coronavirus covid-19 showing the spike protein. A recent study performed a glycosylation analysis of SARS-CoV-2 protein.

Glycans assist in protein folding and help the virus avoid immune recognition by the host. Glycosylation can also have an impact on the antigenicity of the virus, as well as potential effects on vaccine safety and efficacy. Mass spectrometry is widely used for viral characterization studies of influenza viruses. Specifically, mass spec has been used to study influenza protein glycosylation, antigen quantification, and determination of vaccine potency.

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Reflections on Virtual Conference Life: Can You Hear Me?

Today’s guest blog is written by Research Scientist Danette Daniels, PhD.

Virtual Conference Presentation

One of my favorite things about being a scientist is attending conferences. They are an opportunity to connect with the broader community, share ideas, talk about the future, and get inspired. After a conference I would return to my lab feeling so energized and excited, being motivated to push the research forward and work on the next stories we could share.

In March of this year, I remember my shock of watching the conferences slowly getting canceled one by one. First, it was everything in March and April, then May, then through to August.  I was in denial at the time, thinking this would be temporary and that we would all be back together in person in a few months. Then it became quite clear this would not be the case and events were transitioning to a virtual format.

Virtual! I was so skeptical. How could you connect with anyone at a virtual conference? How are people going to ask questions after a talk? How will it be to give a talk basically to your computer, not knowing who is listening, or more importantly for me, not being able to read the audience? I was not looking forward to it, but the alternative, no conferences at all, I thought was worse.

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3 Tips for Preserving Muscle and Joint Health at Work

Today’s guest blog was written by Claire Checovich, Exercise & Ergonomics Specialist in the Promega Wellness Center.

The human body is amazing – it can climb towering cliffs, run hundreds of miles, and move many times its own weight.

It can also be annoying – how many of us have been injured just by sleeping or sitting in a funny position?

We’ve almost certainly all experienced the latter, whether we’re hunched over books and papers or staring at a computer for hours on end. That’s where ergonomics and biomechanics comes in.

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Young Scientist Discovers Potential Anti-SARS-CoV-2 Drug Molecule

14-year-old Anika Chebrolu spent the early months of the COVID-19 pandemic identifying a potential anti-SARS-CoV-2 drug candidate. Originally, she was screening potential anti-influenza treatments, but as she watched COVID-19 case numbers rising around the world, she pivoted to focus instead on the SARS-CoV-2 virus. Several months later, Anika not only discovered a strong candidate for further testing, but she earned the title of 2020 Top Young Scientist in a competition sponsored by 3M.

3d model of coronavirus SARS-CoV-2
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Three Pillars of ESI Mastery: Part One

“If You Only Knew Who You Were…”

Today’s blog is written by Malynn Utzinger, Director of Integrative Practices, and Tim Weitzel, ESI Architect.

We are a little nervous writing about Emotional & Social Intelligence (ESI) “mastery.” This makes it sound like we think it is possible to become perfect at emotional and social intelligence when our actual position on the matter is more about progress than perfection. 

We’re reminded of a quote from a wise teacher. Upon turning 90 he was asked, “What’s one of the most important lessons you have learned in your 90 years?” He replied, “That we are all a mixed bag.” 

No one is perfect. We all have strengths and we all have areas to grow, and we all always will. But the progress we make and its impact on our lives is so worth the effort.

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Just What Is an RLU (Relative Light Unit)?

This post was contributed by guest blogger, Scott Messenger, Technical Support Scientist 2 at Promega Corporation.

It’s always an exciting time in the lab when you find a new assay to answer an important research question. Once you get your hands on the assay, it is always good to confirm it will work for your experimental setup. Repeating the control experiment shown in the technical manual is a great way to test the assay in your hands.

After running that first experiment of your assay, it looks pretty good. The trends of control and treatment are consistent. Time to get on with the experiments…but wait—the RLUs (Relative Light Units) are two orders of magnitude lower than the example data! I can’t show this data to my colleagues; it doesn’t match. What did I do wrong?

Relative Light Units, Measuring, Luminescence

This is a concern that we in Technical Services hear frequently. The concern is real, and I had this same thought when doing some of my first experiments using luminescence. When a question like this comes in, a Technical Service Scientist will make sure the experiment was performed as we described, and in most cases it is. We then start talking about RLUs (Relative Light Units).

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Increasing Testing Efficiency with Multiplexed Detection of SARS-CoV-2 and Influenza A and B

In the Northern hemisphere, the cold and flu season is about to start. Most years that means people schedule flu shots, dust off chicken soup recipes and stock up on tissues. If they start to feel sick, they stay home for a day or two, drink hot tea, eat warm soup and—for the most part— go on with their lives. 

This is not, however, most years. This year the world is battling a pandemic virus, SARS-CoV-2. Symptoms of COVID-19, the disease caused by this virus, mirror those of the flu and common cold, and that overlap in symptoms is going to make life more complicated. Most years, a mild cough or minor body aches wouldn’t even warrant a call to the doctor. This year these, and other undiagnosed cold- and flu-like symptoms, won’t be easily ignored. They could mean kids have to stay home from school, and adults have to self-quarantine from work, for up to 2 weeks. In years past people might have been comfortable treating their symptoms at home, this year people will want answers: Is it the flu? Or is it COVID-19?

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