Finding the Right Maxwell® RSC Kit for Your Nucleic Acid Extraction

This blog was written by guest writers Paraj Mandrekar (Technical Services Scientist 3) and Michelle Mandrekar, (Research Scientist 4).

Here are some designer’s notes comparing the Maxwell® RSC Blood DNA and the Maxwell® RSC simplyRNA kit chemistries for nucleic acid extraction.

The Maxwell RSC Blood DNA Kit and Maxwell RSC simplyRNA Blood Kit were both developed from the same non-silica-based purification chemistry and use the same underlying paramagnetic particle. This chemistry is characterized by an extreme binding capacity (the capacity of nucleic acid that can be bound on the particle), leading to both chemistries being capable of isolating large amounts of nucleic acid volumes and then eluting into relatively small volumes (50 µL). It is not unusual with either chemistry to have isolates that exceed 100 ng/µL. Although the chemistries have several similarities, there are some important distinctions between how the two chemistries were designed that influence which kit you choose for your nucleic acid extraction.

Image of blood with molecules of DNA and RNA superimposed Nucleic Acid Extraction
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Linking Evolution to Pregnancy Outcomes: A Study in Gene Expression

Many of the traits characteristic to human pregnancy are unique. In contrast to other mammals, human pregnancy and labor last longer, and humans are more prone to complications, including infertility, preeclampsia and preterm birth. Research recently published in eLife Sciences by Vincent Lynch, PhD, and colleagues explores the history of gene expression in the human uterus, how it differs from other mammals and how changes in expression may be implicated in our susceptibility to disease.

This study is part of the emerging field of evolutionary medicine, where researchers apply modern evolutionary theory to help us understand the mechanisms behind human health and disease. By studying the history of gene expression, researchers and physicians can illuminate the pathways through which evolution has guided the development of modern tissues and organ systems, and how these systems may differ in one species versus the next.

DNA helix. We review a recent publication that explores the history of gene expression in the human uterus
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How to Get Real-Time Kinetic Data With GloMax® Microplate Readers

Understanding how a compound or drug affects cellular pathways often requires measuring kinetic changes over an extended period of time—from several hours to days. Live-cell kinetic cell-based assays that measure cell viability, cytotoxicity, apoptosis and other cellular pathways are great for collecting real-time data. You don’t necessarily need expensive equipment to run these types of assays. In the videos below, Dr. Sarah Mahan, a research scientist at Promega, demonstrates how you can easily get great 24-hour or multi-day kinetic data using a GloMax® Microplate Reader.

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COVID-19 Intranasal Vaccines Revisited: Can They Reduce Breakthrough Infections?

COVID-19 cases are now being identified primarily among unvaccinated individuals, according to data from the US Centers for Disease Control and Prevention (CDC). However, there has been increasing concern about so-called breakthrough infections among fully vaccinated individuals, particularly after the emergence of the SARS-CoV-2 Delta variant.

COVID-19, sars-cov-2

What is a breakthrough infection? The CDC defines it as “the infection of a fully vaccinated person.” The key finding remains that people with breakthrough infections are still far less likely to experience severe COVID-19 symptoms, in contrast with unvaccinated people; hence the importance of vaccination.

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Finding the Latest Science News While On the Go

Today’s guest blog was written in collaboration with Melissa Martin, a former global marketing intern with Promega. She is a senior at the University of Wisconsin-Madison where she is double majoring in zoology and life sciences communication, with a certificate in environmental studies.

Have you ever found yourself wondering what the newest advancements were on genetically engineering plants or using artificial intelligence in biotechnology but didn’t know where to start looking? You most likely know the basic science behind the headlines, but a general web search may lead to dramatized articles that focus more on getting attention than being accurate. Or you might find a scholarly article that will offer in-depth, peer-reviewed information but may require more time to read than you are willing to give.

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The Latest Addition to the Lab: A Review of the Spectrum Compact CE System

When it comes to acquiring new equipment, choosing the right instrument for your lab can be daunting―you want to make a worthwhile investment that will go the distance, both in longevity and overall capacity. In a perfect world, the instruments available to you would have been thoroughly tested and reviewed, especially as they compare to one another, making your job that much easier.

In the case of benchtop capillary electrophoresis (CE) instruments, researchers Nastasja Burgardt and Melanie Weissenberger have done just that. Their article, titled “First experiences with the Spectrum Compact CE System”, appeared in the International Journal of Legal Medicine and offered a comprehensive review of the performance of the recently released Spectrum Compact CE System in a forensic genetics laboratory setting.

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Using Databases to Find Scholarly Sources

Today’s guest blog was written in collaboration with Melissa Martin, a former global marketing intern with Promega. She is a senior at the University of Wisconsin-Madison where she is double majoring in zoology and life sciences communication, with a certificate in environmental studies.

Peer-reviewed papers are considered the most technical and in-depth way to learn about research and scientific advances. As a student or scientist, you will not only want to read scholarly articles to learn about what others are doing in your field but also to expand your knowledge and learn about scientific advances in completely new areas of study. With countless disciplines of science covering wide-ranging topics such as cell biology, physical chemistry or human behavior, it can be overwhelming to do a general search and find articles and journals that will have the topics relevant to your interests.

Young woman searching databases for findingscholarly articles.
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Resume vs. CV — What Is the Difference?

Today’s guest blog is written by Sophie Mancha, a former global marketing intern with Promega. She is in her 4th year as a PhD candidate in the Biomedical Engineering Department at the University of Wisconsin-Madison, studying pancreatic cancer.

Whether you are applying for a scholarship or trying to land a position in a research lab, there are plenty of decisions to make. Arguably, the most critical consideration is what documents to include to showcase that you are the right person for the job. Specifically, should you be preparing a curriculum vitae (CV) or a resume? What exactly is the difference?

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Feeling Festive with Ion Channels

The tight embrace of welcoming hugs, the cozy warmth of a crackling fireplace, the brisk chill of afternoon walks in snowy woods—these are some of the feelings that, for me, make the winter holidays one of the best times of the year. This season, I’m also choosing to be thankful for the biology that makes these sensations possible.

This year’s Nobel Prize in Physiology and Medicine went to two scientists who discovered the receptors that allow us to sense touch and temperature. Joining other sensory mechanisms recognized by the Nobel committee, these discoveries add to our knowledge of how we interact with the world around us.

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Writing Successful Grants: Putting It Into Words

This blog is part four of a four-part series on grant writing inspired by resources published by the National Institutes of Health (NIH) and others. 

After a long and arduous journey, you have finally arrived at the last stage of your quest to create the world’s most inspiring grant proposal: writing it all down.  

One of the most challenging parts of putting together a grant proposal is simply writing it. You must write to build the reviewers’ confidence in you and demonstrate your contributions to your field, effectively illustrating your qualities as a researcher and your capacity to achieve your goals.

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