Elevate Your Research: Exploring the Power of 8-Dye STR Chemistry with the Spectrum Compact CE System

In genetic research, staying at the forefront of technology is crucial. The latest breakthrough in human identification comes in the form of 8-dye Short Tandem Repeat (STR) chemistry. This innovation promises unprecedented precision and accuracy in DNA analysis, revolutionizing the way we approach genetic studies. In this blog post, we’ll delve into the world of 8-color chemistry and explore how it seamlessly integrates with the game-changing Spectrum Compact CE System.

Understanding 8-Dye STR Chemistry

The introduction of 8-dye chemistry expands the capability of STR analysis, enabling researchers to analyze more DNA markers with smaller amplicons, providing more robust data from degraded or inhibited DNA samples.  The performance of the 8-color dye chemistries from Promega on the Spectrum Compact CE System is sensitive, with both chemsitries (PowerPlex® 35 GY System and the upcoming PowerPlex® 18 E System) producing 100% profiles from their suggested inputs down to as little as 62.5 pg of DNA. The 18E system produced 100% profiles down to 31.25 pg of input DNA with minimal signal bleed through and low system noise.

Table showing percent STR profiles generated with decreasing input DNA using the PowerPlex 35GY or PowerPlex 18 E chemistry on the Spectrum Compact CE System
Table showing percent profiles generated with decreasing input DNA using the PowerPlex® 35GY or PowerPlex® 18E chemistry on the Spectrum Compact CE System.
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Custom in vitro Transcription Reagents for Manufacturing RNA Therapeutics

Doctor filling syringe

Research into vaccines based on RNA began decades ago when scientists theorized that they could harness RNA to produce viral proteins within a cell, prompting a protective immune response. RNA vaccine research drew scientists’ attention during the development of SARS-CoV-2 vaccines during the COVID-19 pandemic, which opened the door for research targeting other diseases with RNA-based therapeutics.

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The R&D Flex Team: A Unique Research Scientist Opportunity

Sarah Mahan embraces change. In fact, she doesn’t just embrace it, she seeks it out, running towards change with arms wide open. 

“If I could do a different thing every week, I would. That’s what my job would be.”

Sarah made her dream a reality when she began leading the Promega R&D Flex Team. This group of diverse research scientists moves around Kornberg Center, contributing resources to accelerate the development of technologies like Lumit Immunoassays and PowerPlex chemistry. They don’t specialize in any field or technology, but rather are constantly challenged to learn new skills quickly. Everywhere they go, they help R&D teams generate more data, answer more questions, and deliver results in less time.

“In short,” Sarah says, “we’re helping research teams make better products, faster.”

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Insights from our Second Annual Targeted Protein Degradation Symposium

In the opening remarks of our second annual Targeted Protein Degradation Symposium, Tom Livelli, VP of Life Sciences Products & Services at Promega, posed a question to the attendees: “What do you want to be able to do today that you can’t?” This aspirational question set the tone for an event where building connections to advance the study and application of proximity-induced degradation took center stage.

More than 90 attendees from academia and industry gathered September 20–21 for the two-day symposium, which was hosted in our inspirational Kornberg Center—the R&D heart of Promega. Through engaging talks, a poster session, “Learn n’ Burn” challenges and social gatherings, participants had the opportunity to reinforce existing collaborations and to connect with others who are making an impact in the field of targeted protein degradation.

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World Mental Health Day: Prioritizing Wellness in the Workplace

Celebrated annually on October 10th, World Mental Health Day provides an opportunity for people and communities to improve knowledge, raise awareness, and mobilize efforts in support of mental health.

Many people find that working is beneficial for their mental health. Having a job can help provide financial stability, establish a structured routine, improve one’s sense of identity, and more. However, at times your work may negatively impact your mental health and vice versa. Read some tips on how you can enhance your workplace well-being and cultivate a happier professional life.

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Glowing Testimonies: A Review of NanoLuc® Use in Model Organisms

NanoLuc®

Model organisms are essential tools in the pursuit of understanding biological processes, elucidating the mechanisms of diseases, and developing potential treatments and therapies. Use of these organisms in scientific research has paved the way for groundbreaking discoveries across various fields of biology. In particular, non-mammalian models can be valuable due to characteristics such as rapid life cycles, low cost, and amenability to use with advanced genetic tools, including bioluminescent reporters such as NanoLuc® Luciferase.

NanoLuc® is a small (19.1 kDa) luciferase enzyme originating from deep sea shrimp that is 100x brighter than firefly or Renilla luciferase. It utilizes a furimazine substrate to produce its bright glow-type luminescence. In the decade following its development, the NanoLuc® toolbox has expanded to include NanoBiT® complementation, NanoBRET™ energy transfer methods, and new reagents such as the Nano-Glo® Fluorofurimazine In Vivo Substrate (FFz) which was designed for in vivo detection of NanoLuc® Luciferase, NanoLuc® fusion proteins or reconstituted NanoBiT® Luciferase. In addition to the aqueous-soluble reagents increased substrate bioavailability in vivo, with fluorofurimazine, NanoLuc® and firefly luciferase can be used together in dual-luciferase molecular imaging studies.

Here we spotlight some recent research that demonstrates how the expanded NanoLuc® toolbox can be adapted to use in non-mammalian models, shedding new light on fundamental biological processes and advancing our understanding of complex mechanisms in these diverse organisms.

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Promega qPCR Grant Series #2: Molecular Biologist, Laura Leighton

Our second installment of the Promega qPCR Grant Recipient blog series highlights Dr. Laura Leighton, a trained molecular biologist and postdoctoral researcher at the Australian Institute for Bioengineering and Nanotechnology. Leighton’s scientific journey features a passion for molecular biology and problem-solving. Her path has been illuminated by mentorship, relationships with fellow scientists and a commitment to creativity in overcoming challenges. Here, we explore her scientific journey, reflect on research lessons and foreshadow her plans for the Promega qPCR grant funds.

Dr. Laura Leighton grew up in a rural area in Far North Queensland, Australia, where she spent her early life exploring critters on the family farm. Her upbringing was infused with a deep connection to the environment, from raising tadpoles in wading pools to observing wildlife and witnessing food grow firsthand. Observing the biology around her ultimately piqued her interest in science from a young age. She then began her academic journey in 2011 at the University of Queensland, Australia. She studied biology while participating in a program for future researchers, which led her to undergraduate research work in several research labs.  She dabbled in many research avenues in order to narrow in on her scientific interests all while adding different research tools to her repertoire.

After serving as a research assistant in Dr. Timothy Bredy’s lab, she decided to continue work in this lab and pursue a PhD in molecular biology. During her PhD, Leighton worked on several projects from cephalopod mRNA interference to neurological wiring in mice. The common thread in these projects is Leighton’s passion for the puzzles of molecular biology:

“I also love molecular engineering and the modularity of molecular parts. There’s something really special about stringing together sequence in a DNA editor, then seeing it come to life in a cell,” she says.

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Promega Fall Art Showcase Honors Truman Lowe, Ho-Chunk Artist and Mentor

Truman Lowe, Ho-Chunk artist and mentor, pictured with Headdress (1989)

Outside the BioPharmaceutical Technology Center, the wind snakes through the tall prairie grasses, drying slowly in the crisp September air. The walking paths through the woods are turning orange with fallen leaves, and the resident sandhill cranes, a fixture of summer at Promega Madison, will soon be heading to their winter home in southern Florida.

Inside the BTC, the Promega Fall Art Showcase is honoring the life of Truman Lowe, an acclaimed Ho-Chunk artist whose sculptural works evoke a powerful connection with nature. For decades, Lowe was a professor in the Department of Art at the University of Wisconsin-Madison where he was an exceptional mentor to young artists. He was known for encouraging artists to fearlessly delve into their artistic voice and equipping them with the necessary tools to navigate the art world. The Fall Art Showcase honors his legacy by exhibiting his art alongside pieces by several former students.

Truman Lowe: Visionary Artist, Mentor and Teacher

Truman Lowe was born on the Ho-Chunk Nation of Wisconsin reservation in 1944. He recounted a childhood of drawing with rocks on the Black River and creating crafts like baskets and beadwork with his parents. Though he loved art from an early age, Lowe says that he never thought of art as a profession until he was studying for his undergraduate art degree at the University of Wisconsin-LaCrosse. There, he became fascinated with Michelangelo, who inspired him to realize that art could be “a profession as well as a passion.”1

Visitors to the Promega Fall Art Show can see sculptures by Truman Lowe alongside works by several of his students.

After earning a graduate degree and moving through several teaching positions at the high school and university level, Lowe accepted a position as Native American studies coordinator and assistant professor of art at UW-Madison in 1975. This began a 45-year tenure in the department of art, where he fostered a deep understanding and appreciation for Native American art and culture among his students. He also served as a curator of contemporary art at the National Museum of the American Indian.

Lowe is known for large, site-specific installations that use natural materials including wood, stone and metal. His works push creative boundaries and exhibit a unique blend of versatility, precision and emotional depth. Lowe’s sculptures have been exhibited around the world, from museums including the Metropolitan Museum of Art to embassies in Bolivia and Cameroon. A sculpture titled Effigy: Bird Form was displayed on the White House grounds during the Clinton administration and was recently reinstalled atop Observatory Hill at UW-Madison, close to the former site of Native American effigy mounds. 

Lowe, who died in 2019, was a beloved mentor to many students over his long tenure at UW-Madison. A university-published obituary quotes John Hitchcock, professor and Associate Dean at UW-Madison, saying, “Truman encouraged us to stay strong as artists and to our vision as makers.” The widespread love of Lowe will be on display at the Promega Fall Art Showcase, where six of his former students will be exhibiting alongside Lowe’s own works.

Promega Fall Art Showcase

The Fall Art Showcase opened on September 19 with a symposium featuring guest speakers Patricia marroquin Norby and Jo Ortel. Norby is the Associate Curator of Native American Art at the metropolitan Museum of Art, and the first person of Indigenous descent hired for a full-time curatorial position in the museum’s 150-year history. Jo Ortel is an author, art historian and Professor Emerita of Art History of Beloit College. Ortel is also the author of a notable biography of Truman Lowe titled “Woodland Reflections: The Art of Truman Lowe.”

The Promega Culinary Team collaborated with Chef Elena Terry of the culinary organization Wild Bearies to offer traditional Ho-Chunk food at the reception. Chef Terry provided recipes and connected the team with indigenous purveyors to source ingredients. Promega also collaborated with Little Eagle Arts Foundation to include pieces of Ho-Chunk culture into the event.

The Fall Art Showcase runs through December 29 and is open to the public Monday through Friday 8:00 am – 4:00 pm at the Promega BioPharmaceutical Technology Center. For more information, visit https://www.promega-artshow.com/

1As quoted in Woodland Reflections: The Art of Truman Lowe by Jo Ortel


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LinkedIn for Early Career Scientists: Advice from Promega’s Interns

Laptop computer displaying logo of LinkedIn, an American business and employment-oriented service that operates via websites and mobile apps

In today’s world of social networking, LinkedIn has emerged as the clear winner for professionals in all industries. With its powerful networking capabilities and innovative career development features, LinkedIn has revolutionized how individuals connect, collaborate and advance their careers.

In this blog you will hear from some of Promega’s interns as they share valuable advice for early career scientists looking to expand their network, establish meaningful connections and propel their career forward.

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Leveraging LinkedIn: A Guide for Early Career Scientists

female holding iPhone with LinkedIn screen. LinkedIn helps build portfolio and look for a new job

As an early career scientist, you may have already realized that the key to a successful career is not just an impressive resume or CV, but a strong professional network. In today’s interconnected digital age, there is no better platform to build this network than LinkedIn. With more than 930 million users worldwide, LinkedIn is a powerful tool for connecting with professionals in your industry, exploring job opportunities, and building your personal brand. 

In this blog, I’ll cover everything you need to know to establish a strong presence on LinkedIn and achieve your professional goals.

Creating a Strong Profile

Your profile can either make or break your success on LinkedIn. A well-crafted profile has the potential to create lasting impressions and open doors to new career and networking opportunities. Below are a few tips to help you create a profile that is sure to impress potential connections and employers:

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