Understanding how a compound or drug affects cellular pathways often requires measuring kinetic changes over an extended period of time—from several hours to days. Live-cell kinetic cell-based assays that measure cell viability, cytotoxicity, apoptosis and other cellular pathways are great for collecting real-time data. You don’t necessarily need expensive equipment to run these types of assays. In the videos below, Dr. Sarah Mahan, a research scientist at Promega, demonstrates how you can easily get great 24-hour or multi-day kinetic data using a GloMax® Microplate Reader.
How to Get 24-Hour Kinetic Data
- Pre-warm instrument.
- Set up assay plate with cells and reagent.
- Select preinstalled protocol on the GloMax® Instrument and set interval.
- Add test compound to cells.
- Place plate in the instrument and start run.
How to Get Multi-Day Kinetic Data
- Select preinstalled protocol on the GloMax® Instrument and pre-warm instrument.
- Set up plate with cells, reagent and test compounds.
- Cover plate with gas-permeable membrane and put in incubator.
- Place plate in the GloMax® Instrument and read.
- Put plate back into incubator until next time point.
Learn more about our GloMax® Microplate Readers or request a demo!
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Johanna is a Science Writer at Promega. She earned her PhD in Biomedical Sciences at Baylor College of Medicine. She was a freelance writer and full-time mom for five years before joining Promega. Johanna is from Taiwan and she believes Taiwanese food is the best in the world. She loves doing yoga, traveling and spending time with her two kids.
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