The Lighter Side of Science

Happy Holidays from Promega Connections

Posted on by Promega

cookingYour Promega Connections Bloggers will be taking a break from December 23, 2014–January 5, 2015, to retool and gather up our thoughts so that we can cook up New Year of intriguing science posts. You can look forward to posts about bioluminescence, cytotoxicity and cell culture, kinases and cellular signaling networks, epigenetics, and the new twists and turns in biotechnology that are making their way into our labs and our lives. We will continue our tradition of posts to guide the job seekers and those of you in career transitions, and of course we will provide lots of technical tips and tricks for your work at the bench.

If you have any burning technical questions or a science or technology topic on which you would like to see a post, let us know in the comment section below.

Be sure you are subscribed to the RSS feed so that you can join us on our blogging journey in 2015.

Happy New Year!

What Things Are You Thankful for in Science?

Posted on by Michele Arduengo

What are you thankful for in science?
What are you thankful for in science?
As the social media lead for Promega, I keep my eye on trends in new media. I have personal accounts that I keep mostly to see what other people are doing. I try hangouts, social networking and other things so that I have an idea of developing practices outside of the biotechnology industry. One activity that has been popular over the last couple of years during the month of November in the United States is the Facebook post of “30 days of thanksgiving”.

I wondered what “thanksgiving” looks like to the research scientist. So I asked:

What are the things you are thankful for in science?

The answers have been as varied as the people I talked to ranging from little things like water bath floats to really big things, like the renewal of your research funding or achieving tenure.

Here are some of the answers from my informal inquiries:

“Tube floaties for water baths.”

—E.V., genomics product manager

“I was always thankful for Geiger counters.”

—K. G., science writer

“Thermal cyclers and Taq Polymerase. As an undergrad I watched someone sit with a timer and move their tubes between water baths at 3 different temperatures, opening tubes and adding polymerase at the end of each cycle. Modern PCR is SOOO much easier.”

—M.M., research scientist

“I am thankful for competent cells. I remember preparing the CaCl2 and doing slow centrifugation. Also thankful for serum-compatible transfection, rapid ligations and online journal access (no longer have to traipse over to the university library to get papers photocopied- uuurrrgggghhh).”

—R.D., technical services scientist

“How about T-vectors for cloning? I was no molecular biologist, but could make a T-vector work.”

—K.K., science writer

“I am thankful for open-access journals and the ability to read the full article without an institutional subscription.”

—S.K., science writer

“I am ever so thankful for ONLINE ORDERING! So awesome. Throw in online technical manuals, on-line support tools, on-line calculators – all are awesome!!”

—A.P., director, scientific courses

“I am thankful for automated sequencing- manual sequencing was laborious and hazardous!!!”

—R.G., technical services scientist

Do any of these resonate with you? What are you thankful for as a scientist? Let us know in the comments.

Use of Mass Spectrometry to Quantitate Food Allergens

Posted on by Gary Kobs

 

Food allergies are becoming increasingly prevalent among children. Credit: James Gathany, CDC
Food allergies are becoming increasingly prevalent among children. Credit: James Gathany, CDC

Food allergies are increasing worldwide and becoming a public health issue, especially among children are concerned. Children have a higher prevalence of food allergies, with about 4–8%, compared to adults (1–5%).  Currently antibody-based methods such ELISA (enzyme-linked immunosorbent assay) are the primary method for food allergen analysis. In most cases antibodies are only available for single well-known allergens. Often those that are commercially available are poorly characterized resulting cross-reactivity that leads to false-positive results in diagnostic tests.

A recent publication (1) presented a review of an alternative technology based on mass spec (i.e., multiple reaction monitoring, MRM) that circumvents the drawbacks of antibody based methods. MRM allows precise quantitative determination of target proteins in complex samples with broad dynamic range.  MRM also provides quantification of different isoforms. It is noted that tryptic digestion followed by mass spec analysis, has already identified several unique peptides for different allergens, including those found in crustaceans, eggs, fish, peanuts, soy and wheat. In summary the challenge is now to select the appropriate tryptic signature peptide(s) for the respective allergen and to develop well characterized standards (i.e., isotope labeled standards) to ensure accurate quanititation.

Citation:

Koeberl, M et al. (2014) Next Generation of Food Allergen Quantification using Mass Spectrometric Systems J. Proteome Research  13, 3499–509.

She’s Going Soft! – A commentary on “hard” and “soft” sciences

Posted on by Karen Reece

scientific-methodThis week I gave notice that I would be terminating my employment at Promega. This was a very difficult decision as I have really enjoyed the past six years here.  While I am leaving Biotech, I will not be leaving science all together.  Over the past few years, I have used my research, analytical, and organizational skills to assist various non-profit organizations in the community.  My primary focus will be on reform of the criminal justice system and racial disparities.  Spreading the word about this decision has resulted in a number of responses (overwhelmingly positive) including the comment that I am going soft! This got me thinking about where the terms hard and soft science came from. Continue reading “She’s Going Soft! – A commentary on “hard” and “soft” sciences”

Grays On the Move: Whale Watching in San Diego

Posted on by Robert Deyes

As a boy, one of my favorite childhood books was without a doubt, Herman Melville’s Moby Dick. For those not familiar with the story, it tells of the obsessive quest of one Captain Ahab to kill a white whale in revenge for an attack that left him with a severed leg. The story is told by the character Ishamel who accompanies Ahab and provides the reader with a front row seat on the doomed saga of Ahab and his crew. After reading the book, I set myself the task of learning more about whales and how we can do more to protect the lives of these magnificent ‘leviathans of the deep’. My parents bought me Jacques Cousteau’s Whales one Christmas. From that moment on, my heart and mind were transfixed. This year I was privileged to see Gray whales for the first time, following their migratory path down the west coast of the United States. Together with a handful of other excited tourists, I went on a 3 hour cruise outside of San Diego bay, organized by the Scripps Institute Birch Aquarium. Below are several of the many pictures I took on that memorable day.

All Aboard Skipper!
All Aboard Skipper!

Continue reading “Grays On the Move: Whale Watching in San Diego”

Ghosts

Posted on by Sam Jackson
buddhabrot fractal image
Formless, recursive and abstract, it can be tough to wrestle the ghosts of the conscious mind.

Please believe me when I say this is the hardest thing I’ve done. Typing this sentence might as well be lifting a boulder, and the next could be even heavier. Before this, the hardest thing I’d done was say “good morning” to co-workers, and before that, it was simply getting out of bed.

Just about the only thing I find easy is going to bed, but sleeping is a different story. Every night I lie down, unsure if I’ll fall asleep within seconds and wake what seems like moments later, swatting aimlessly at my alarm clock, or if I’ll remain awake, tired beyond belief but some mysterious finger in the dyke preventing a flood of sleep from washing over me.

I’m one of the approximately 21 million people in the United States who suffer from major depression. Let me tell you, it’s kind of a bummer. Lying awake at night might sound terrible, but it’s the easiest thing in the world compared to writing a sentence, saying “hello”, smiling. I live each day negotiating a watery fog, often unsure what people tell me, confused about what comes next, and desperate for the energy to participate in the world.

This isn’t an essay asking for sympathy; receiving pity from others would only make me feel worse. Besides, as a function of suffering from depression, I’m convinced nobody is reading this, that nobody is going to read this. This essay is for me. Only by engaging and grappling with this disease in words and in actions can I ever hope to pin it to the ground.

Continue reading “Ghosts”

5 Signs You’re Ready to Earn that Ph.D.

Posted on by Karen Reece

confused5. No one in your family bothers to ask you what you’re studying anymore.

The longer you spend in a Ph.D. program the more opportunities your family has to ask you what you’re doing in school. No doubt, you’ve spent the first couple years of graduate school going to family functions and trying to explain to your grandma what a molecule is. She will eventually come up with an explanation of what you are doing that she can share with her friends. Her description of your work may or may not be correct, but she’s not going to bother trying to understand it anymore. “Good for your honey, you’re so smart!”

4.  Your former life as a bartender or grocery store clerk starts to sound really appealing. Continue reading “5 Signs You’re Ready to Earn that Ph.D.”

Confessions of a Cookie Mom

Posted on by Sarah Dewbre

I decided last year that I would be a troop leader for my daughter’s Girl Scout Daisy troop. I thought it would be a great way to spend some one-on-one time with her, while volunteering for a cause I believe in. It started off positive; I met with the other leader, we had all these great ideas for what we would do! This was going to be so fun! Well, time went on, and the meetings seemed longer and longer;  the craft ideas were getting much less creative, and by the end of the year the girls were playing tag for the last 15 minutes of the meetings because we had run out of things to do. I realized that I really am not the best “traditional” troop leader, but I still wanted to be involved in a leader capactiy. I know! I will be the cookie coordinator for the Girl Scout cookie season! I can use the skills I am much better at (organization, metrics), and not have to be creative or crafty. Perfect!

Maybe I could arrange them into a futon frame or bookshelf?
Maybe I could arrange them into a futon frame or bookshelf?

We are two weeks into cookie season, and I would like to give the job back. I am completely obsessed with cookies. I think about them almost all day; I dream about them at night. I am placing cookie orders, counting cookies, schlepping cookies, EATING cookies. Here is a picture of my family room. This is what it looks like all the time. I have a “cookie spreadsheet”. I have nightmares that it will get accidentally deleted somehow, and that would throw my life into a tailspin. Continue reading “Confessions of a Cookie Mom”

Fun with Chemistry for Valentine’s Day

Posted on by Sara Klink
Image credit: Morguefile

Looking for last-minute gift ideas? Wondering what chemistry has to do with Valentine’s Day? The chemists of the American Chemical Society (ACS) have curated a web page called Valentine’s Day Chemistry that offers gift ideas (for example, make a crystal heart using pipe cleaners, borax and hot water), explains the chemistry behind chocolate and flowers, shows a video that equates chemical bonding to people interacting at a party and more. The links and videos offer something for everyone, whether you want to have fun with friends and family of all ages or just want to learn something new about chemistry.

Happy Valentine’s Day!