The Lighter Side of Science

Get Ready to Celebrate Pi!

Posted on by Michele Arduengo

26562342_lWhat will you be doing on 3/14/15 at precisely 9:26:53? Twice the clocks will align with the first few digits of my favorite irrational and transcendental number (3.141592653…) or π. Over 1 trillion digits to the right of the decimal point have been calculated, and they still go on, never making a pattern, never repeating.

Take any circle, that blueberry pie that your grandmother baked would be a good choice. Measure the circumference and divide by the diameter and you will have Pi, the mathematical constant that represents the ratio of circumference to diameter in a circle. You probably encountered Pi in your early mathematics education when learning the formula for the area of a circle (2πr2).

In celebration of Pi, here are a few Pi diversions to brighten your day. Continue reading “Get Ready to Celebrate Pi!”

Neuroscience Explains Harry Potter’s Appeal

Posted on by Terri Sundquist

BookWithGlassesCurling up with a good book is one of life’s greatest pleasures, whether you’re reading on a tropical beach while on vacation or nestled into your favorite chair at home. As your eyes skim over the words, your mind conjures up images of the events unfolding on the page. Books can take us to fantastic places, real and imaginary, that we will never visit in our lifetime. And while there is some pleasure to be gained from nonfictional books, my favorite books all seem to fall in the realm of fiction. I am not alone. The science fiction and fantasy genre of literature continues to be one of the most popular. Why do so many readers find these types of books so enticing and engaging?

It all comes down to science, specifically neuroscience.

Continue reading “Neuroscience Explains Harry Potter’s Appeal”

Biotechnology Ice Breakers: A Few Conversation Starters

Posted on by Amy Prevost

quiz pictureThe biotechnology industry is one of the most dynamic out there – in fact, it never stands still! For non-scientists this can be intimidating. For scientists, it can be challenging to explain what we do in ways that non-scientists can understand and appreciate.

Scientists have made great strides in improving our ability to use molecular processes to our advantage, from discovering the basics of how to isolate and manipulate DNA to gaining an understanding of how genes direct the creation of proteins in cells.  It’s clear that there is a lot we can contribute to the scientific literacy of the general public.

In this spirit, we’ve designed a short quiz for both non-scientists (you may learn something new) and scientists (you may find it useful for engaging in conversations with your non-scientist friends and family members).  Spoiler alert: answers are provided. Continue reading “Biotechnology Ice Breakers: A Few Conversation Starters”

Happy Holidays from Promega Connections

Posted on by Promega

cookingYour Promega Connections Bloggers will be taking a break from December 23, 2014–January 5, 2015, to retool and gather up our thoughts so that we can cook up New Year of intriguing science posts. You can look forward to posts about bioluminescence, cytotoxicity and cell culture, kinases and cellular signaling networks, epigenetics, and the new twists and turns in biotechnology that are making their way into our labs and our lives. We will continue our tradition of posts to guide the job seekers and those of you in career transitions, and of course we will provide lots of technical tips and tricks for your work at the bench.

If you have any burning technical questions or a science or technology topic on which you would like to see a post, let us know in the comment section below.

Be sure you are subscribed to the RSS feed so that you can join us on our blogging journey in 2015.

Happy New Year!

What Things Are You Thankful for in Science?

Posted on by Michele Arduengo

What are you thankful for in science?
What are you thankful for in science?
As the social media lead for Promega, I keep my eye on trends in new media. I have personal accounts that I keep mostly to see what other people are doing. I try hangouts, social networking and other things so that I have an idea of developing practices outside of the biotechnology industry. One activity that has been popular over the last couple of years during the month of November in the United States is the Facebook post of “30 days of thanksgiving”.

I wondered what “thanksgiving” looks like to the research scientist. So I asked:

What are the things you are thankful for in science?

The answers have been as varied as the people I talked to ranging from little things like water bath floats to really big things, like the renewal of your research funding or achieving tenure.

Here are some of the answers from my informal inquiries:

“Tube floaties for water baths.”

—E.V., genomics product manager

“I was always thankful for Geiger counters.”

—K. G., science writer

“Thermal cyclers and Taq Polymerase. As an undergrad I watched someone sit with a timer and move their tubes between water baths at 3 different temperatures, opening tubes and adding polymerase at the end of each cycle. Modern PCR is SOOO much easier.”

—M.M., research scientist

“I am thankful for competent cells. I remember preparing the CaCl2 and doing slow centrifugation. Also thankful for serum-compatible transfection, rapid ligations and online journal access (no longer have to traipse over to the university library to get papers photocopied- uuurrrgggghhh).”

—R.D., technical services scientist

“How about T-vectors for cloning? I was no molecular biologist, but could make a T-vector work.”

—K.K., science writer

“I am thankful for open-access journals and the ability to read the full article without an institutional subscription.”

—S.K., science writer

“I am ever so thankful for ONLINE ORDERING! So awesome. Throw in online technical manuals, on-line support tools, on-line calculators – all are awesome!!”

—A.P., director, scientific courses

“I am thankful for automated sequencing- manual sequencing was laborious and hazardous!!!”

—R.G., technical services scientist

Do any of these resonate with you? What are you thankful for as a scientist? Let us know in the comments.

Use of Mass Spectrometry to Quantitate Food Allergens

Posted on by Gary Kobs

 

Food allergies are becoming increasingly prevalent among children. Credit: James Gathany, CDC
Food allergies are becoming increasingly prevalent among children. Credit: James Gathany, CDC

Food allergies are increasing worldwide and becoming a public health issue, especially among children are concerned. Children have a higher prevalence of food allergies, with about 4–8%, compared to adults (1–5%).  Currently antibody-based methods such ELISA (enzyme-linked immunosorbent assay) are the primary method for food allergen analysis. In most cases antibodies are only available for single well-known allergens. Often those that are commercially available are poorly characterized resulting cross-reactivity that leads to false-positive results in diagnostic tests.

A recent publication (1) presented a review of an alternative technology based on mass spec (i.e., multiple reaction monitoring, MRM) that circumvents the drawbacks of antibody based methods. MRM allows precise quantitative determination of target proteins in complex samples with broad dynamic range.  MRM also provides quantification of different isoforms. It is noted that tryptic digestion followed by mass spec analysis, has already identified several unique peptides for different allergens, including those found in crustaceans, eggs, fish, peanuts, soy and wheat. In summary the challenge is now to select the appropriate tryptic signature peptide(s) for the respective allergen and to develop well characterized standards (i.e., isotope labeled standards) to ensure accurate quanititation.

Citation:

Koeberl, M et al. (2014) Next Generation of Food Allergen Quantification using Mass Spectrometric Systems J. Proteome Research  13, 3499–509.

She’s Going Soft! – A commentary on “hard” and “soft” sciences

Posted on by Karen Reece

scientific-methodThis week I gave notice that I would be terminating my employment at Promega. This was a very difficult decision as I have really enjoyed the past six years here.  While I am leaving Biotech, I will not be leaving science all together.  Over the past few years, I have used my research, analytical, and organizational skills to assist various non-profit organizations in the community.  My primary focus will be on reform of the criminal justice system and racial disparities.  Spreading the word about this decision has resulted in a number of responses (overwhelmingly positive) including the comment that I am going soft! This got me thinking about where the terms hard and soft science came from. Continue reading “She’s Going Soft! – A commentary on “hard” and “soft” sciences”