General

Promega Spain Gets Moving Outdoors For A Cause

Posted on by Karen Burkhartzmeyer

A bike ride through the beautiful countryside, or even a routine walk to the corner store, is good for both body and soul. But sometimes even that’s not enough to get us moving in the fresh air. So our Promega Biotech Ibérica branch recently found a way to raise the stakes, and make outdoor activity even more valuable for its employees.

Promega Biotech Ibérica collected images of the beautiful Spanish countryside during their Kilometros Solidarios campaign.
Promega Biotech Ibérica collected images of the beautiful Spanish countryside during their Kilometros Solidarios campaign.

Last summer, the branch came up with the idea to donate one euro to the children’s oncology department of Hospital La Paz in Madrid for every kilometer its staff biked, walked or ran outside. The idea behind Kilometros Solidarios (Solidarity Kilometers) was simple but powerful: healthy activity leading directly to the health of others.

The plan worked. During the months of July, August and September, employees got up and outside and the branch raised €1884 (about $1993 USD) toward the purchase of a much-needed portable ultrasound machine for the hospital. (To point out the obvious, 1884 kilometers of outdoor activity were racked up by just 15 employees. That’s 4710 times around an Olympic track, or about the same distance as walking, biking or running from Promega Ibérica to Promega GmbH or from the Promega Madison headquarters straight south to the Gulf of Mexico.) Continue reading “Promega Spain Gets Moving Outdoors For A Cause”

To Seq, or Not to Seq

Posted on by Nicole Sandler

Seq—shorthand for “sequence”— has become a more recognizable term thanks to a novel and provocative genomics initiative called the BabySeq Project. The project, officially launched in May 2015, was designed to explore the impact of whole-exome sequencing (WES) on newborn infants and their families. A randomized, controlled trial to sequence healthy and sick infants and then provide sequencing information, it is the first of its kind. Those infants randomized to receive WES undergo genetic sequencing of all protein-coding genes and analysis of about 1,700 genes implicated in childhood health, along with 18 years of follow up genetic counseling.29813751-nov-2-blog-post-nicole-600x470-web

The project is directed by Robert C. Green, geneticist and physician at Brigham and Women’s Hospital, Harvard Medical School and the Broad Institute, and Alan H. Beggs of Boston Children’s Hospital and Harvard Medical School. Funding, totaling $25 million, comes from the National Institute of Child Health and Development and the National Human Genome Research Institute. Continue reading “To Seq, or Not to Seq”

A Very Happy and BATTY Halloween

Posted on by Michele Arduengo
Crowds gather on the Congress Ave Bridge in Austin, TX to see the bat colonly.
Crowds gather on the Congress Ave Bridge in Austin, TX to see the bat colony.

My colleagues in the scientific communications group at Promega are pretty sure that I have bats in my belfry. And, they may be right. After all I have written extensively and repeatedly about bats in North America and the threat that they are facing from White Nose Syndrome, the devastating disease caused by a cold-loving fungus (you can read my last post here). And, just last week I skipped an awesome party on Rainey Street in Austin, TX, to instead hang out by the Congress Ave bridge in hopes of seeing the urban bats fly.

But just in time for the annual Promega Connections Halloween blog, I stumbled across some good news for our distant mammalian cousins.

Continue reading “A Very Happy and BATTY Halloween”

Royal Lessons for Scientific Discovery

Posted on by Robert Deyes

rdeyes-2This Summer I had the chance to travel to Isle Royale (‘Royal Island’) in Lake Superior for what was a trip that would challenge me in more ways than one.  Our group, comprising 3 adults and 5 boys, drove up to Copper Harbor in the Michigan Upper Peninsula and then made the four hour ferry crossing to the Isle Royale National Park.   We spent the next 6 days hiking across the island, covering a total of 50 miles with backpacks and tents weighing us down every step of the way.

The sights were fantastic, the weather tremendous and the sheer thrill of being unreachable by text, email or phone thoroughly satisfying to say the least.  But, like any adventure of this nature, there was an element of fear that accompanied the trip.  What if anyone got hurt on the island?  How would we handle losing all our food if critters raided our camp during the night?  Might someone get seriously sick on the ferry crossing?  Had we brought enough food to keep us healthy while not overloading our back packs with unnecessary weight that would hinder our progress?  What if our portable water filters got clogged up? Continue reading “Royal Lessons for Scientific Discovery”

Research Teams Demonstrate Bivalent Binding of a Novel Bromodomain Protein Inhibitor

Posted on by Promega
13305818-cr-da-nanoluc-application_ligund

Today’s blog is written by guest blogger Kristin Huwiler from our Cellular Analysis and Proteomics Group.

Two research collaborations, one in Europe and a second in the US, have just published in Nature Chemical Biology (1,2) on the identification of BET inhibitors (bi-BETs) that bind via a bivalent mechanism to both bromodomains of BRD4. These bivalent chemical inhibitors exhibit high cellular potency and affinity relative to their monovalent predecessors. By developing high-affinity ligands that engage both bromodomains simultaneously within BRD4, the authors illustrate a concept that may be applicable in the development of selective, potent ligands for other multi-domain proteins. Here we review the work presented in the Waring et al. paper using the Promega NanoBRET™ Technologies to characterize the mechanism of action of their bivalent probe.

The bromodomain and extraterminal (BET) sub-family are some of the most studied bromodomain-containing proteins (3). The BET subfamily of proteins contain two separate bromodomains. BRD4 is one well studied member of the BET sub-family. Several small molecule inhibitors that target BRD4 have been developed as potential therapeutics for various cancers with promising initial studies, but to date are all monovalent, binding each bromodomain of the BET family members separately (2).

Continue reading “Research Teams Demonstrate Bivalent Binding of a Novel Bromodomain Protein Inhibitor”

Of Elephant Research and Wildlife Crime – Molecular Tools that Matter

Posted on by Nicole Sandler

Here at Promega we receive some interesting requests…

Take the case of Virginia Riddle Pearson, elephant scientist. Three years ago we received an email from Pearson requesting a donation of GoTaq G2 Taq polymerase to take with her to Africa for her field work on elephant herpesvirus. Working out of her portable field lab (a tent) in South Africa and Botswana, she needed a polymerase she could count on to perform reliably after being transported for several days (on her lap) at room temperature. Through the joint effort of her regional sales representative in New Jersey/Pennsylvania (Pearson’s lab was based out of Princeton University at the time) and our Genomics product marketing team, she received the G2 Taq she needed to take to Africa. There she was able to conduct her experiments, leading to productive results and the opportunity to continue pursuing her work.

Continue reading “Of Elephant Research and Wildlife Crime – Molecular Tools that Matter”

The Birth of a Disease? A New Anthrax-Like Disease Found in Sub-Saharan Africa

Posted on by Kelly Grooms

Copyright
Copyright

It usually starts with one; one dead animal, one sick individual, one case that a doctor thinks is unusual. These are all ways that a new disease makes its presence known. In the case of Bacillus cereus biovar anthracis, it started with a dead chimpanzee (1).  

The wild western chimpanzee was found dead in Côte d’Ivoire in 2001. An investigation led by scientists from the Robert Koch Institute in Berlin identified the pathogenic cause of death to be an atypical B. cereus isolate that caused an anthrax-like disease. Continue reading “The Birth of a Disease? A New Anthrax-Like Disease Found in Sub-Saharan Africa”

Exploring the Land of the Silver Fern—Part II

Posted on by Promega

In 2014, Promega created a special incentive to reward field science consultants who help the scientific community take advantage of our on-site stocking program. The winners had to meet ambitious criteria to receive 2 round-trip tickets to anywhere in the world, a week of paid vacation and spending money. Our four winners from 2014 will share photos and stories about their journeys in a semi-regular Friday feature on the Promega Connections Blog.

Today’s travelogue is Part II of the adventures of Sarah Theos, a client support consultant, who used her award to travel to New Zealand. You can read Part I here.

Day 7: Queenstown to Te Anau

My husband surprised me that morning by booking a massage appointment at the spa to soothe my aching muscles. It was a rainy morning and we had a long drive ahead of us to the town of Te Anau, the stopover town on the way to the Fiordland National Park and Milford Sound.  We also saw that we had lucked out on our itinerary as it was supposed to be a beautiful day in Milford the next day (a place that gets over 300 days of rain each year).  Therefore, we didn’t mind one more day of rain if it meant the next day would be amazing.  We drove through the Canterbury plains, passing tons of sheep and dairy farms along the way.  We also found it interesting that they farm deer in NZ.  We drove past a lot of deer farms.  When we arrived in Te Anau, we grabbed some food and went to the local grocery store to stock up on snacks and more wine.  New Zealand has a booming wine industry and we thought that it was worth sampling as many wines as we could.  The day was gray and cold but we decided to walk along Lake Te Anau anyway.  It is a beautiful, massive lake but we didn’t see very much of the surrounding mountains because of the fog.  We stopped briefly at the bird sanctuary to see the Kaka, the Crested Grebe, and other beautiful birds.  That evening, we had the most amazing 5 star dinner at the Redcliff Café and loved it so much that we booked a table there for the next evening!  Since we were getting up super early the next day to drive to Milford, we went to bed early.

Day 8:  Drive through the Fiordland to Milford Soundnew-zealand1a

This had to be my most favorite day of the entire trip.  Not only was the weather amazing, the entire drive to and from Milford Sound was mind blowing.  The New Zealanders call Milford the 8th Natural Wonder of the World and it is easy to see why.  It is in a remote part of the island and 99% of the Fiordland will never see a human.  The Fiordland has waterfalls aplenty, sweeping, colorful landscapes with millions of gorgeous lupin flowers and gigantic, snow covered mountains.  It truly feels as though you have entered another world.  We left early so we would avoid the dreaded crowds and tour buses that clog the two lane road from 10am-4pm. We stopped constantly as the views just kept getting better and better around every twist and turn.  One notable stop was The Chasm.  The Chasm is where the raging Cleddau River has worn a path straight through the mountain so it virtually disappears into the rock.  There are many massive, perfectly round pothole formations in the limestone where pebbles have worn their way through the rocks.  We were the only two people there at that time of the morning.  It felt majestic just to stand there and listen to the tremendous sound of the powerful river disappearing into the mountain and reappearing on the other side. Continue reading “Exploring the Land of the Silver Fern—Part II”

Magnetic Bacteria Carry Drugs into Tumors

Posted on by Isobel Maciver
cancer cell

At first glance, the biology of magnetic, underwater-dwelling, oxygen-averse bacteria may seem of little relevance to our most pressing human health problems. But science is full of surprises. A paper published in Nature Nanotechnology presents an inspired use of these bacteria to deliver anti-cancer drugs to tumors, specifically targeting the oxygen-starved regions generated by aggressively proliferating cells.

Continue reading “Magnetic Bacteria Carry Drugs into Tumors”

Easy Automated Genomic DNA Isolation for GMO Testing: From Vision to Reality

Posted on by Kelly Grooms
29980616-July25-PureFood---Kelly-600x300

The European Union (EU) has a zero tolerance policy for products containing any material from non-authorized genetically modified (GM) crops. Seed entering EU markets may not contain even trace amounts of non-authorized genetically modified material. In 2012, as the global use of GM crops increased, seed testing loads in the EU continued to build. Isolating genomic DNA (gDNA) using traditional manual methods was becoming impractical in the face of increasing amounts of material that required testing. There was a growing need for an automated method to isolate gDNA from seed samples. Working to address this need, a group of scientists from the Bavarian Health and Food Safety Authority collaborated with scientists from Promega Corporation to evaluate the Maxwell® 16 Instrument and the associated chemistry as possible a solution for the testing labs.

Continue reading “Easy Automated Genomic DNA Isolation for GMO Testing: From Vision to Reality”