An Ode to Packaging Engineers

Comic by Ed Himelblau.

In my second or third year as a graduate student, I had to ship some microfluidic masters to a collaborator in Kenya. The masters were extremely fragile and took me several days in a cleanroom gown to make. I was horrified at having to send them on a perilous journey overseas, and somewhat flabbergasted that they made it to Nairobi whole and well. And yet, every day thousands of delicate items zoom around the world and arrive at their destinations in one piece. How?

A couple months ago, I visited the lab where our packaging engineers (yes, that’s a thing) do their work. Here’s what I learned. Continue reading “An Ode to Packaging Engineers”

Hey, iGEMers! We’re talking to you!

The 2018 iGEM Giant Jamboree is upon us! This Wednesday, October 24th, thousands of you will flood into Boston, weighed down by posters and presentation materials, but energized by the excitement of a non-stop science-packed conference. Promega will also be attending, with a booth full of helpful giveaways and staff standing by to answer all your questions about science, Promega or future careers. As you make your final plans for the Jamboree, here are a few helpful tips for making the most of this incredible opportunity.

Continue reading “Hey, iGEMers! We’re talking to you!”

What Could You Do with a Faster, More Consistent ADCC Reporter Bioassay?

Fc receptor-mediated antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) by which antibodies target diseased cells for elimination. Traditional methods for measuring ADCC require primary donor peripheral blood mononuclear cells (PBMCs) or purified natural killer (NK) cells that express Fc receptors on the cell surface. Killing of target cells is an endpoint of this pathway activation and is used in classic ADCC bioassays.

PBMCs and NK cells are notoriously difficult to isolate and culture. Furthermore, cultured cells can be a source of variability.

There is a Better Way

Watch this video to learn why traditional ADCC assays can be problematic. You’ll also learn a solution. Find out how  to not only save time but also reduce assay variability.

For more details on the benefits of working with ADCC Reporter Bioassays go to the product page.

There you’ll see how standardized reagents in Promega ADCC Reporter Bioassays ensure better results and better consistency in an ADCC Reporter Bioassay that saves you time.

My City Flooded, and There’s More to Come

It seemed like the rain was never going to stop. It started in the morning, and when I left work around 5pm, it was still coming down hard. I took my normal route home through a back country road. As I turned right onto Fitchrona Road, a long line of cars came into view. There’s usually some congestion leading to the stop sign ahead. Except today, something was different. About 20 yards of the road ahead was submerged in water. Continue reading “My City Flooded, and There’s More to Come”

Moving Towards Zero Hunger, One Genome at a Time

Farmer and a pile of cassava bulbs.

Have you ever thought about plant viruses? Unless you’re a farmer or avid gardener, probably not. And yet, for many people the battle against agricultural viruses never ends. Plant viruses cause billions of dollars in damage every year and leave millions of people food insecure (1–2), making viruses a major barrier to meeting the United Nations’ global sustainable development goal of Zero Hunger by 2030.

At the University of Western Australia, Senior Research Fellow Dr. Laura Boykin is using genomics and supercomputing to tackle the problem of viral plant diseases. In a recent study, Dr. Boykin and her colleagues used genome sequencing to inform disease management in cassava crops. For this work, they used the MinION, a miniature, portable sequencer made by Oxford Nanopore Technologies, to fully sequence the genomes of viruses infecting cassava plants.

Cassava (Manihot esculenta) is one of the 5 most important calorie sources worldwide (3). Over 800 million people rely on cassava for food and/or income (4). Cassava is susceptible to a group of viruses called begomoviruses, which are transmitted by whiteflies. Resistant cassava varieties are available. However, these resistant plants are usually only protected against a small number of begomoviruses, so proper deployment of these plants means farmers must know both whether their plants are infected and, if so, the strain of virus that’s causing the infection.

Continue reading “Moving Towards Zero Hunger, One Genome at a Time”

In Defense of Wild Spaces in the Yard

Pale purple asters and milkweed. Copyright S. Klink.

Surrounding my mowed lawn is a wild, mostly uncultivated space that currently has goldenrod blooming with tall asters starting to blossom. Every day when I pass these flowers, I see bumblebees, butterflies and other insects collecting the nectar to eat or store for the winter. Last year, when a section of soil was disturbed during construction of a building, I decided to seed the area with native wildflowers rather than grass. (I am not a fan of mowing the lawn.) Watching the series of flowers bloom over the late spring to autumn has been beautiful, colorful and full of tiny moments of joy. Not only do I see insects enjoying the flowering plants, but birds will land on the taller greenery, sometimes just resting, sometimes collecting seeds. I am not sure who has been startled more often, me or the birds when I walk by, flushing a bird from the thicket of tall plants.

Monarch butterfly on thistle photographed in the prairie at Promega headquarters in Madison, WI. Copyright Promega Corporation.

Where some people might see wild, unruly areas, I see Monarch butterflies on their daily flight, fluttering above me and the “weeds”. I have even been lucky enough to find Monarch caterpillars munching on milkweed, a common plant in my wild space. Despite my efforts, I have a lot of tall ragweed appearing in my yard, but have discovered that birds love the seeds, including my chickens, and squirrels will remove and eat the leaves. In addition, I see fireflies in early June through late August, many I find hanging out on the shady greenery during the day before their light display at night. Continue reading “In Defense of Wild Spaces in the Yard”

Quantitating Kinase-Inhibitor Interactions in Live Cells

Kinase target engagement is a new way to study kinase inhibitors for target selectivity, potency and residency. The NanoBRET™ TE Intracellular Kinase Assays enable you to quantitate kinase-inhibitor binding in live cells, making these assays an exciting new tool for kinase drug discovery research.

For today’s blog about NanoBRET™ TE Intracellular Kinase Assay, we feature spokesperson Dr. Matt Robers. Matt is part of Promega’s R & D department and is one of the developers of the NanoBRET™ TE Intracellular Kinase Assay.

Continue reading “Quantitating Kinase-Inhibitor Interactions in Live Cells”

Fished to the Edge: How DNA Identification Can Help Fight the Illegal Trade of Threatened Shark Species

Whether your first encounter was peering through the thick glass of an aquarium tank or peeking through your fingers in a darkened theater, there is something about sharks that captures our imagination. These fierce, and sometimes fearsome, creatures have existed in our oceans for over 400 million years, and  survived multiple mass extinction events, including the one that killed the dinosaurs. They are not, however, the vicious, vengeful villain that some movies would have us believe. Sharks are apex predators, who play an important role in the world’s ocean ecosystem by regulating the population of prey species below them.  Unfortunately, they are also part of one of the most threatened group of marine fish in the world. Of the more than 400 species of sharks that exist in our oceans today, approximately 15% are considered vulnerable, endangered or critically endangered. Continue reading “Fished to the Edge: How DNA Identification Can Help Fight the Illegal Trade of Threatened Shark Species”

Beneath the Writing: Non-Invasive DNA Sampling from Modern and Historic Writing Surfaces

We can learn a lot about the past and its people from the written records of the time. What people write and how they write it can gives us glimpses into historical events, interpersonal relationships, social standing and even social and cultural norms. From paper to papyrus to clay tablets, the surface that holds the writing can tell us things that the words cannot.

For plant-based writing surfaces, the quality of the surface or even the technique used to make it can give historians and archeologists insight into the people who used them. What more could we learn if we knew what plant, or plants, were used in the production of ancient writing material? Continue reading “Beneath the Writing: Non-Invasive DNA Sampling from Modern and Historic Writing Surfaces”

Factors Influencing Compound Potency in Biochemical and Cellular Assays

Late in 2017, a group here at Promega launched an exciting new assay, the NanoBRET™ Target Engagement (TE) Intracellular Kinase Assay.

It’s easy for me to call this assay exciting; I was an editor on the project team. But judging by the reviews on the SelectScience® web site, others are excited about NanoBRET™ Target Engagement Intracellular Kinase Assay too.

A review of the NanoBRET TE Kinase assay from SelectScience® .
A review of the NanoBRET TE Kinase assay from SelectScience® .
Continue reading “Factors Influencing Compound Potency in Biochemical and Cellular Assays”