For most molecular biology applications, knowing the amount of nucleic acid present in your purified sample is important. However, one quantitation method might serve better than another, depending on your situation, or you may need to weigh the benefits of a second method to assess the information from the first. Our webinar “To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation” given by Doug Wieczorek, one of our Applications Scientists, discussed three methods for quantitating nucleic acid and outlined their strengths and weaknesses.
Continue reading “Methods for Quantitating Your Nucleic Acid Sample”Author: Sara Klink
Successful Ligation and Cloning of Your Insert
You have PCR amplified your insert of interest, made sure the PCR product is A tailed and are ready to clone into a T vector (e.g., pGEM®-T Easy Vector). The next step is as simple as mixing a few microliters of your purified product with the cloning vector in the presence of DNA ligase, buffer and ATP, right? In fact, you may need to consider the molar ratio of T vector to insert.
Continue reading “Successful Ligation and Cloning of Your Insert”Can We Have Healthy Bees and Green Lawns?
Some people like a perfectly green carpet of grass; I welcome the biodiversity of clover, dandelions and other weeds (although I could do without the painful thistles). Of course, I also notice many pollinating insects including bumblebees seem to enjoy visiting the flowers that bloom in the lawn. However, concerns have arisen over the use of insecticides and the health of our pollinators. There has been extensive talk about the collapse of honeybee colonies and declining populations of native pollinators like bumblebees. One area of research is the use of insecticides, especially neonicotinoids, and how they affect bees but most studies were done in lab environments, not in a lawn where the bees would collect nectar from flowering plants. Larson, Redmond and Potter decided to study both clothianidin, a neonicotinoid insecticide, and chlorantraniliprole, an anthranilic diamide, and examine how the insecticides affected bumblebees that foraged on treated lawns.
Neonicotinoids are systemic insecticides that are potent selective agonists of nicotinic acetylcholine receptors in insects, and are applied as sprays or granules during the spring for control of root-feeding grubs and other pests. Anthranilic diamides are a new class of insecticides that activate insect ryanodine (calcium channel) receptors and cause lethal paralysis in sensitive species. Chlorantraniliprole shows low acute bee toxicity, a promising development, but this anthranilic diamide has not been tested in the field.
Continue reading “Can We Have Healthy Bees and Green Lawns?”Ancient Samples Confirm the Cause of the 6th Century Plague Pandemic
When I started writing about research on Yersinia pestis and the Black Death, I was amazed at the ability to recover 14th century bacterial DNA from human remains, show Y. pestis was the caustive agent of the Black Death and then sequence the strain to compare to modern Y. pestis strains. The publications I read always mentioned the three waves of pandemics that devastated human populations in the introduction, and the Black Death was not the oldest one. The putative first pandemic was the Plague of Justinian in the 6th century, named after the Byzantine emperor. Like with the Black Death, there is debate about whether Y. pestis is the causative agent of the Plague of Justinian. The research published in PLOS Pathogens built on earlier work to isolate and genotype the suspected Y. pestis causative agent from human remains in 6th century graves, but this time with more stringent protocols enacted to answer critics who questioned the authenticity of earlier results.
Continue reading “Ancient Samples Confirm the Cause of the 6th Century Plague Pandemic”Peering into the History of Leprosy
What is it about the UK and graves and dead people to identify or bacteria to sequence? First, there are Black Death graves, then Richard III under a car park and now a leprosy grave site. While we do know leprosy has been a scourge of human beings for centuries, tracking the different strains and origins of the disease is relatively new. Taylor et al. were interested in this grave site near Winchester, located in southern Britain, north of Southampton and southwest of London, because little is known about the disease organism Mycobacterium leprae during this early medieval period (late 11th to 12th century). What could they learn about the strains that caused leprosy and the possible spread of the disease? Continue reading “Peering into the History of Leprosy”
It Isn’t Spring Until the Birds Arrive
With spring finally gaining a foothold in the upper Midwest as temperatures rose above 60 degrees Fahrenheit this weekend, everyone including myself has been spending more time outdoors. Like my fellow blogger Karen, I am a gardener and have been digging and planting everything from raspberries to currants to apple and peach trees.
However, one of my favorite parts of spring is hearing and seeing all the birds that flit around my property. Ironically, I was outside in a hat, gloves and coat when I heard my first redwing blackbirds of the season but since then, robins have taken up residence, and the house sparrows have returned, stealing the grass seed I scattered on my rather thin lawn. Nuthatchers are entertaining to watch as they peck at tree bark upside down while they hunt for food. An eastern bluebird has taken up residence nearby, and I enjoy spotting him every day, sitting in a tree or resting on my shepherd’s hook. The chickadees are fun to spot as they switch branches on a tree. Crows occasionally stop by especially if I have left out some pizza crusts for them. Turkeys have even hung around my front lawn, and I startled them (and they, me) as I opened my front door and they quickly flew away. I was up early one morning before most birds were active and all I could hear was “gobble, gobble, gobble”.
I’m still learning about all the different birds that stop over on my property as I expect there are more I have not identified. Which birds do you enjoy spotting in the spring?
A Molecular Approach to Estimating Time of Death
I will admit that over the years, I have watched various crime scene investigation shows and read several books by Kathy Reichs and Patricia Cornwell because I was fascinated by forensic science. These same books and shows are a guilty pleasure because as a scientist, I know these portrayals do not accurately reflect how laboratory work is done. Answers are not so cut and dried as an exact estimation of time of death—for example, death was five hours before the body was found in an abandoned warehouse. However, scientists are always looking for ways to improve accuracy in time of death estimates, which are currently based on a few physical clues that are affected by environment and other factors. One approach taken by Sampaio-Silva et al. (1) was to assess the RNA degradation using reverse transcription quantitative PCR (RT-qPCR).
The authors of this PLOS ONE article wanted to determine if nucleic acid degradation could be used as a method to improve time of death estimates. Continue reading “A Molecular Approach to Estimating Time of Death”
Mummies are a Reservoir of Viral History
Smallpox was a disease caused by infection with one of two strains of Variola virus (Variola major and Variola minor) and a worldwide scourge that killed up 35% of the people it infected. Luckily, a vaccine was developed when Edward Jenner noticed milkmaids infected with cowpox did not contract smallpox. While Jenner was not the first to vaccinate against smallpox, his discovery and testing were spread to a wide audience and thus became the basis for the vaccination efforts that have eradicated the virus in our lifetime. Despite all the research on smallpox, not much is known about the evolution of the virus. Sequence data for the virus only span the last 50–60 years. However, recent efforts published in the New England Journal of Medicine uncovered a new source for examining the history of smallpox infection: mummies.
Continue reading “Mummies are a Reservoir of Viral History”How to Make Plastic Out of Milk
As the daughter of a (former) dairy farmer, I love milk and all its derived dairy products (e.g., cheese and butter). However, it wasn’t until my colleague Michele highlighted a kid science app that I realized milk is a great science medium as well. In fact, I recently discovered mixing vinegar with milk will create moldable plastic. Not only is this milk-derived product fun for kids and adults, but it also offers a history lesson: the resulting substance was used before petroleum-based plastic was available. Watch the video to learn about a fun kitchen experiment and potential handmade gift opportunity all in one package.
[youtube=http://www.youtube.com/watch?v=VFvik_THcNQ]
The Healing Power of Yogurt
There has been much made of probiotics regulating digestive health in the news and repeatedly hawked by people sharing the benefits of yogurt with special bacteria that will change your life. I was advised by my mother that when I was taking antibiotics, eating yogurt would help counteract some of the negative consequences of oral antibiotics and repopulate my gastrointestinal tract with beneficial bacteria. But I was unaware of research published in Bioscience, Biotechnology, and Biochemistry showing that yogurt with Lactobacillus gasseri could actually heal acid-induced ulcers—at least in rats. Continue reading “The Healing Power of Yogurt”