Author: Promega

Top Ten Uses for Pumpkin

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In honor of Halloween, here is a Top Ten Uses of Pumpkin list for your enjoyment:

10. Means of transportation on the high seas or emergency flotation device (pumpkin boats; see the video).

9. Elementary Math Lessons. Determine circumference and radius. How much does a pumpkin weigh? Estimate the number of seeds. Check out pumpkin math ideas here.

8. Cholesterol-lowering snack. After ruthlessly scooping out the innards of a pumpkin, clean the seeds, bake them in the oven and enjoy your healthy snack. Pumpkin seeds contain phytosterols, compounds that that have been shown to reduce levels of LDL cholesterol, as well as magnesium, a mineral needed in the diet.

7. Physics Laboratory. Pumpkin Chuckin’ Contest (video).

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Cell-Free Protein Synthesis

Posted on by Promega

Cell-free protein synthesis (aka: in vitro translation) refers to protein production in vitro using lysates that provide the cellular machinery necessary for synthesis. Ribosomes, tRNAs, aminoacyl-tRNA synthetases, initiation/elongation/termination factors, GTP, ATP, Mg2+ and K+ are among the requirements for a translation system. These are provided by lysates, which can be from prokaryotic or eukaryotic sources, depending on your requirements.

Cell-free protein synthesis is most commonly used for generating protein for study of things like:

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