I was blasting a holiday music playlist while driving recently, and Presley’s Blue Christmas played. I couldn’t get the phrase “Christmas Cloning Blues” out of my mind, and by the time I arrived at my destination, this happened:

Cloning Blues Christmas

(to the tune of Blue Christmas by Elvis Presley)

I’ll have a blue Christmas without you

Colonies so blue, insert without you

Incubating my plates at 37 degrees

Won’t be the same if you’re not in lacZ

And all those blue colonies are forming

When my lab mates’ clonings are performing

They’ll be doing alright,

With their plates all filled with white

But I’ll have a blue, blue, blue cloning

They’ll be doing alright

But my insert’s not in sight

And I’ll have a blue, blue, blue cloning

Your writers at Promega Connections wish you nothing but successful clonings, PCR and cell culture this season—wherever your experiments take you. If you are having technical issues or just want some background before you begin, we have many resources to help you troubleshoot your experiments.

• Blue/White cloning blog with tips for success
• Cloning a PCR insert? We have blogs to guide you.
  • This blog on T-Vector Cloning is filled with tips and answers to commonly asked questions
  • This blog has even more answers to frequently asked questions about T-Vector cloning.
  • Need to put blunt-ended DNA into a T-Vector?
• Our Subcloning Guide on our website has advice on all things cloning to help you avoid the cloning blues.
• Our Student Resource Center has information to cover your entire workflow…from isolating DNA to PCR to Cloning to Downstream Analysis.
• More of a visual learner? That’s great. The Promega YouTube channel has an entire playlist covering everything cloning.
• Finally, Promega has amazing technical services scientists who can help you plan, troubleshoot and even celebrate your successes. Stumped and can’t find the answers on the website? Ask a technical services scientist for help.

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Michele Arduengo

Supervisor, Digital Marketing Program Group at Promega Corporation

Michele earned her B.A. in biology at Wesleyan College in Macon, GA, and her PhD through the BCDB Program at Emory University in Atlanta, GA where she studied cell differentiation in the model system C. elegans. She taught on the faculty of Morningside University in Sioux City, IA, and continues to mentor science writers and teachers through volunteer activities. Michele manages the digital marketing program team at Promega.

Latest posts by Michele Arduengo (see all)

• Unlocking the Secrets of ADP-Ribosylation with Arg-C Ultra Protease, a Key Enzyme for Studying Ester-Linked Protein Modifications  - November 13, 2024
• Exploring the Respiratory Virus Landscape: Pre-Pandemic Data and Pandemic Preparedness - October 29, 2024
• From Fins to Genes: DNA Barcoding Unlocks Marine Diversity Along Mozambique’s Coast - October 15, 2024